Pittsburgh quarterback Eli Holstein was carted off the field with 5:32 left in the first quarter with an apparent left ankle injury during Saturday’s Atlantic Coast Conference game against host Louisville.

The freshman was sacked at the Panthers’ 49-yard line by Louisville’s Ashton Gillotte, who rolled on the quarterback’s ankle. Holstein was in a walking boot as he was helped to the cart.

Holstein missed last week’s game against Clemson after suffering a head injury in the loss to Virginia two weeks ago.

Holstein was 3-for-5 passing for 51 yards and an interception before exiting.

Nate Yarnell, who threw for 350 yards in the loss to Clemson, replaced Holstein.